The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 13, 2004
Filed:
Feb. 20, 2002
Theo T. Nikiforov, San Jose, CA (US);
Caliper Life Sciences, Inc., Mountain View, CA (US);
Abstract
The methods and systems for identifying nucleotides monitor changes in fluorescent signal that occur during the polymerase-mediated extension of dye labeled primer molecules. In certain aspects, the methods monitor changes in a fluorescent signal, e.g., relative polarization, emitted from a reaction mixture that contains a dye labeled primer that hybridizes to a target sequence such that the primer ends at the position of interest. Perfect hybridization at the position results in extension of the primer while imperfect hybridization, e.g., a mismatch at the position of interest results in no extension reaction. The occurrence of extension reaction is monitored by a change in the fluorescent signal of the mixture.