The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 06, 2004
Filed:
Jan. 07, 2000
Simon P. Robins, Inverurie, GB;
Jeffrey D. Brady, Aberdeen, GB;
Quidel Corporation, San Diego, CA (US);
Abstract
Disclosed is a method for measuring a level of pyridinoline and/or deoxypyridinoline in a sample. In the method, a non-hydrolyzed sample containing one or more peptide-bound collagen pyridinium crosslinks selected from the group consisting of pyridinoline, deoxypyridinoline, or both, is contacted with a protease reagent under conditions effective for the protease reagent to cleave the crosslinks from attached collagen amino acids and peptides, so that peptide-bound forms are converted to native, peptide-free pyridinoline and deoxypyridinoline. After proteolysis, the level(s) of native, peptide-free pyridinoline and/or deoxypyridinoline are measured. Preferably, proteolysis is effective to ensure that at least 80% of total pyridinium crosslinks are present as the native, peptide-free forms. The method is particularly useful in screening or monitoring collagen degradation activity. Kits and reagents for use in the method are also disclosed.