The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 23, 2004
Filed:
Jun. 29, 2001
Applicant:
Inventors:
Assignee:
Iowa State University Research Foundation, Ames, IA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/10 ; C12N 1/00 ; C12N 1/15 ; C12N 1/21 ; C12N 1/511 ;
U.S. Cl.
CPC ...
C12N 5/10 ; C12N 1/00 ; C12N 1/15 ; C12N 1/21 ; C12N 1/511 ;
Abstract
Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes, in each of the three possible reading frames, an ATG start codon and a histidine tag, and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences of interest 5′ or 3′ to the 3-frame His-tag DNA sequence. Vectors also are provided with cloning sites for inserting nucleotide sequences of interest 3′ of the ATG start codon and 5′ of the 3-frame His-tag DNA sequence.