The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 24, 2004
Filed:
Feb. 26, 2001
Applicant:
Inventor:
Carsten-Peter Carstens, LaJolla, CA (US);
Assignee:
Stratagene, La Jolla, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/564 ; C12N 1/566 ; C12N 1/509 ; C12Q 1/68 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12N 1/564 ; C12N 1/566 ; C12N 1/509 ; C12Q 1/68 ; C07H 2/104 ;
Abstract
The present invention provides a method of transfer of a gene of interest from a first vector to a product vector comprising contacting a first and second vector in vitro with a site-specific recombinase so as to generate a co-integrate vector comprising the components of the first and second vector, and introducing the co-integrate vector to a prokaryotic host cell so as to generate a product vector by rolling circle replication, comprising the gene of interest.