The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 24, 2004
Filed:
Sep. 17, 1998
Applicant:
Inventors:
Takashi Etoh, Shizuoka, JP;
Mamoru Takahashi, Shizuoka, JP;
Assignee:
Asahi Kasei Pharma Corporation, Tokyo, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/32 ;
U.S. Cl.
CPC ...
C12Q 1/32 ;
Abstract
A method for assaying ammonia and/or ammonium ions contained in a liquid sample which comprises subjecting the sample to a reaction at least in the presence of an NAD synthetase, deamidated NAD, ATP, Mg ions and/or Mn ions, a dehydrogenase capable of forming ammonia molecules from an amine substrate and an oxidized NAD, and the amine substrate, and then determining the amounts of the ingredients consumed or generated by the reaction; and an assaying composition comprising all of the above-specified ingredients. The method is inexpensive and is effective in accurately assaying ammonia and/or ammonium ions present in a few minutes.