The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 03, 2004

Filed:

Feb. 28, 2002
Applicant:
Inventors:

Naoto Matsuyama, Takatsuki, JP;

Mina Fukuhara, Osaka, JP;

Masaharu Takayama, Ibaraki, JP;

Koji Mizuno, Kyoto, JP;

Assignee:

Azwell, Inc., Osaka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/34 ; C12Q 1/37 ; G01N 3/353 ;
U.S. Cl.
CPC ...
C12Q 1/34 ; C12Q 1/37 ; G01N 3/353 ;
Abstract

The present invention provides a method and a kit for detecting or quantitatively determining homocysteine rapidly and simply and with high sensitivity by oxidizing the residual homocysteine cosubstrate, the produced homocysteine-converting enzyme product or an enzyme reaction product thereof in the presence of an SH reagent to produce hydrogen peroxide and determining the produced hydrogen peroxide by color development using an oxidative color-developing agent. By using the method and kit of the present invention, homocysteine in biological samples, in particular, in body fluids such as blood and urine can be detected and quantitatively determined rapidly and simply and with high sensitivity.


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