The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 13, 2004
Filed:
May. 14, 2001
Robert Dicosimo, Rockland, DE (US);
Arie Ben-Bassat, Newark, DE (US);
Robert D. Fallon, Elkton, MD (US);
E. I. du Pont de Nemours and Company, Wilmington, DE (US);
Abstract
A method for preserving immobilized or unimmobilized microbial cells having nitrilase activity and for stabilizing the nitrilase activity of unimmobilized or immobilized microbial cells has been developed. Aqueous suspensions containing at least 100 mM bicarbonate, carbonate, or carbamate salts limit microbial contamination of the stored enzyme catalyst, as well as stabilize the desired nitrilase activity of the unimmobilized or immobilized cells. Microorganisms which are characterized by an nitrilase activity and are stabilized and preserved by this method include 72-PF-15 (ATCC 55747), 72-PF-17 (ATCC 55745), 72W (ATCC 55746), and transformed microbial cells having nitrilase activity, the host cells transformed with 72W nitrilase activity. Especially preferred is an embodiment using ammonium carbamate as the inorganic salt.