The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 30, 2003
Filed:
Aug. 15, 2001
Applicant:
Inventors:
Martin A. Smith, Brookline, MA (US);
Galina N. Fomovskaia, Boston, MA (US);
Mikhail A. Fomovsky, Boston, MA (US);
Neil J. Butt, Cambridge, GB;
Matthew Baker, Ely, GB;
Assignee:
Whatman, Inc., Newton, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12P 1/934 ; C12M 1/34 ; C07H 2/102 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12P 1/934 ; C12M 1/34 ; C07H 2/102 ; C07H 2/104 ;
Abstract
The present invention provide methods for utilizing spent leukodepletion filter devices as a source of material for the isolation and analysis of genomic DNA (gDNA), including polymorphism, genotyping, and pharmacogenomic studies. Cellular retentate with the filter contains leukocytes, which are lysed to release the nuclei. The nuclei are lysed or ruptured to release genomic DNA, which is then isolated and used for subsequent analysis.