The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 23, 2003
Filed:
Nov. 13, 2001
Applicant:
Inventor:
Lars-Erik Peters, Lafayette, CO (US);
Assignee:
Eppendorf AG, , DE;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12Q 1/912 ; C12Q 1/908 ; C12N 9/00 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12Q 1/912 ; C12Q 1/908 ; C12N 9/00 ;
Abstract
Methods for improving sensitivity and specificity of polynucleotide synthesis are disclosed. The method includes reversibly blocking thermophilic polymerase activity with non-nucleic acid polyanions in a temperature dependent manner. The methods control target specific primer extension throughout all stages of a DNA or RNA amplification reaction. Corresponding compositions and kits are disclosed.