The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 16, 2003
Filed:
Jun. 01, 2001
Applicant:
Inventors:
John T. Mulligan, Seattle, WA (US);
John C. Tabone, Bothell, WA (US);
Assignee:
Blue Heron Biotechnology, Inc., Bothell, WA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 3/300 ; C12Q 1/68 ; C12P 1/934 ; C07H 2/102 ; C07H 2/104 ;
U.S. Cl.
CPC ...
G01N 3/300 ; C12Q 1/68 ; C12P 1/934 ; C07H 2/102 ; C07H 2/104 ;
Abstract
Synthetic oligonucleotides, such as synthetic DNA, often contain sequence errors due to synthetic failures (e.g., side products and/or truncated products). Methods are provided herein for improving the sequence fidelity of synthetic double-stranded oligonucleotides by separative depletion of synthetic failures. Separation is effected by utilization of methodologies in a preparative mode under denaturing conditions. A preferred use of the methods relates to gene synthesis.