The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 04, 2003

Filed:

Jan. 20, 1999
Applicant:
Inventors:

Joseph R. Bertino, New York, NY (US);

Emine A. Ercikan-Abali, New York, NY (US);

Debabrata Banerjee, New York, NY (US);

Shin Mineishi, New York, NY (US);

Michel Sadelain, New York, NY (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/20 ; C12N 9/02 ; C12N 1/500 ; C07H 2/104 ; C07K 1/00 ;
U.S. Cl.
CPC ...
C12N 1/20 ; C12N 9/02 ; C12N 1/500 ; C07H 2/104 ; C07K 1/00 ;
Abstract

New mutant forms of human dihydrofolate reductase (DHFR) which have properties superior to the previously disclosed mutants have mutations at both amino acid 22 and amino acid 31. Specific mutant forms are Ser31Tyr22, Ser31Phe22, Gly31Tyr22, Gly31Phe22, Ala31Tyr22 and Ala31Phe22. The mutant DHFR of the invention maybe used as a selectable marker, and to modify the genome of human cells, particularly bone marrow cells or peripheral blood stem cells, to render them resistant to chemotherapy using antifolate agents.


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