The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 09, 2003
Filed:
Mar. 18, 2002
Lawrence Restaino, Elburn, IL (US);
R&F Laboratories, Inc., West Chicago, IL (US);
Abstract
An isolation plating medium for use in processes for the presumptive identification of 0157:H7 from a sample that also contains other strains of The plating medium comprises at least one carbohydrate that 0157:H7 is incapable of fermenting, but other strains of do ferment said carbohydrate, a pH indicator dye that changes the color of the plating medium to a first color when the pH of the medium changes, a chromogenic substrate that reacts to beta-galactosidase to form a precipitate in the plating medium of a second color which contrasts with the first color. Both 0157:H7 and the other strains of are beta-galactosidase producers during metabolism, whereby a microorganism which ferments the carbohydrate but does not produce beta-galactosidase will produce colonies in the plating medium of the first color, 0157:H7 which does not ferment the carbohydrate but produces beta-galactosidase will produce colonies in the plating medium of the second color, and the other strains of which ferment the carbohydrate medium and produce beta-galactosidase will produce colonies in the plating medium of a third color which is the color that results from the mixing of the first and second colors, and a sufficient mass of an agent to solidify the mixture. The invention also includes processes of using the plating medium to identify and enumerate 0157:H7 and other microorganisms.