Microbial enrichment using a container having a plurality of solid support particles

Abstract

A method and device for collection and concentration of microbes via selectively attracting microbes to specific substrates chemically conjugated to a solid surface, such as a particle, are provided. Further, a method for selectively enriching for specific microorganisms using a device for collecting populations of microorganisms from an environmental sample comprising a solid support having a surface for attachment of an enrichment media is provided. The method for selectively enriching one or more populations of microorganisms from an in situ environment includes providing a device which has a container having a plurality of solid support particles in the container and a selective microbial enrichment media chemically attached thereto the particles. The container also has permeable openings for the microorganisms to provide entry of them therein so that the microbes will contact the enrichment media. The microorganisms are collected and concentrated in the device by placing it into the environment and the microorganisms are attracted to it and thereby migrate into the device through the permeable openings. The populations of microorganisms may be enriched in the device with a microbial attractant and growth inhibitor in the media and removed therefrom the device. The microorganisms enriched in the device include extremophiles. The solid support particles include glass, silica aerogel or a combination thereof. Glass beads may also be used as the solid support particles.