The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 12, 2003
Filed:
Jul. 06, 1998
Applicant:
Inventors:
Jan-Gunnar Gustafsson, Uppsala, SE;
Johan Öhman, Upplands-Väsby, SE;
Assignee:
Pharmacia AB, Stockholm, SE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/14 ; C07K 1/446 ; C12N 1/512 ; C12N 1/21 ; C12N 1/500 ;
U.S. Cl.
CPC ...
C07K 1/14 ; C07K 1/446 ; C12N 1/512 ; C12N 1/21 ; C12N 1/500 ;
Abstract
Methods for producing correctly folded biological active recombinant protein or polypeptide comprise the steps of expressing the protein or polypeptide in prokaryotic cells, harvesting the cells, directly solubilizing the cells in a buffer at pH of about 8 to 11 with the chaotropic agent and a reducing agent, and diluting the solution with water and a diluent. The method is conducted in the absence of mechanical disruption of the cells and isolation and washing of refractile bodies.