The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 05, 2003

Filed:

Jul. 21, 2000
Applicant:
Inventors:
Assignee:

Syngenta Mogen B.V., Leiden, NL;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/582 ; C12N 1/584 ; C12N 1/531 ; A01H 1/02 ; A01H 5/00 ;
U.S. Cl.
CPC ...
C12N 1/582 ; C12N 1/584 ; C12N 1/531 ; A01H 1/02 ; A01H 5/00 ;
Abstract

The present invention is directed to the production of male sterile plants by providing them with a recombinant DNA capable of specific expression in the male reproductive system of a plant of the enzyme trehalose phosphate (TPP). Resotration of the fertility can be established either by providing said male sterile plants with a recombinant DNA capable of expression of trehalose phosphate synthase (TPS) under control of an inducible promoter or with a recombinant DNA capable of expression of a suppressor protein which suppresses expression of TPP under control of an inducible promoter. This inducible restoration possibilities enable the maintenance of a homozygous male sterile line. Restoration can also be done by spraying the male sterile plants with gibberellic acid. For production of hybrids or hybrid seed a site-specific recombination system is provided, by inserting two site=specific recombination sites flanking the recombinant DNA coding for TPP and crossing the male sterile lines with lines expressing the corresponding recombinase. By crossing the recombinase will excise the gene coding for TPP and fertile hybrids are produced.


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