The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 29, 2003

Filed:

Mar. 07, 1995
Applicant:
Inventors:

Philip Frederick Brode, III, Cincinnati, OH (US);

Bobby Lee Barnett, Cincinnati, OH (US);

Donn Nelton Rubingh, Cincinnati, OH (US);

Chanchal Kamur Ghosh, West Chester, OH (US);

Assignee:

Procter & Gamble Company, Cincinnati, OH (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/56 ; C12N 1/557 ; C12N 1/574 ; C11D 3/386 ;
U.S. Cl.
CPC ...
C12N 9/56 ; C12N 1/557 ; C12N 1/574 ; C11D 3/386 ;
Abstract

The present invention relates to subtilisin 309 variants having a modified amino acid sequence of wild-type subtilisin 309 amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type subtilisin 309 (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the subtilisin 309 variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type subtilisin 309. The present invention also relates to DNA sequence encoding such subtilisin 309 variants. The present invention also relates to compositions comprising such subtilisin 309 variants for cleaning a variety of surfaces.


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