The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 22, 2003

Filed:

Jan. 16, 2001
Applicant:
Inventors:

Paulo César Peregrino Ferreira, Belo Horizonte MG, BR;

Erna Geessien Kroon, Belo Horizonte MG, BR;

Jenner Karlisson Pimenta Dos Reis, Belo Horizonte MG, BR;

Isabella Bias Fortes Ferraz, Belo Horizonte MG, BR;

Rômulo Cerqueira Leite, Belo Horizonte MG, BR;

Assignee:

Universidade Federal de Minas Gerais, Belo Horizonte MG, BR;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 1/4155 ;
U.S. Cl.
CPC ...
C07K 1/4155 ;
Abstract

The present invention relates to a method and kit for detecting antibodies in clinical samples of animals infected with equine infectious anemia virus using the immunodiagnosis with the recombinant viral antigen p26. The antigen was bound to solid supports (microtitter plates, tubes, beads or nitrocellulose papers or nylon) and reacted with the test serum. After incubation with conjugated anti-equine immunoglobulin-enzyme the reaction was revealed with a solution composed of the substrate of the enzyme used in the conjugate (cromogene). After development of the reaction (color formation) it was stopped with acid solution and measured. The immunoassay may be a direct second antibody immunoassay, a one or two step sandwich immunoassay.


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