The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 24, 2003
Filed:
Jun. 18, 1997
Robert Reid Townsend, Oxford, GB;
Raj Bhikhu Parekh, Oxon, GB;
Sally Barbara Prime, Oxford, GB;
Nick Sinclair Wedd, Oxford, GB;
Oxford GlycoSciences (UK) Ltd, Abingdon, GB;
Abstract
The present invention relates to a method for generating a library of peptides based on a peptide of a predetermined molecular mass and to a method of determining the amino acid sequence of the peptide from the library. A set of amino acids that can be present in the peptide are defined, and an allowed library of all possible sequences of the amino acids in the set having a total mass equal to the predetermined molecular mass, allowing for water lost in forming peptide bonds and for protonation, is generated. The allowed library may be generated by first generating a set of all combinations of amino acids having a total mass equal to the predetermined molecular mass of the peptide and then calculating all linear permutations of all amino acids in each such combination. Generally, the molecular mass is determined using a mass spectrometer. A theoretical fragmentation spectrum for every amino acid sequence in the allowed set may also be calculated for use in determining the amino acid sequence of the peptide. The fragmentation spectra may be of any type known in the art, such as a mass spectrum or a protease or chemical fragmentation spectrum. The theoretical fragmentation spectra calculated for each member of the allowed peptide library may be compared to an experimental fragmentation spectrum for the unknown peptide to determine the amino acid sequence of the unknown peptide.