The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 03, 2003
Filed:
Jun. 02, 2000
Applicant:
Inventor:
Christophe D. Reymond, Prilly, CH;
Assignee:
RMF Dictagene S.A., Epalinges, CH;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/48 ; C12P 2/106 ; C12N 9/12 ; C12N 1/20 ; C12N 1/00 ;
U.S. Cl.
CPC ...
C12Q 1/48 ; C12P 2/106 ; C12N 9/12 ; C12N 1/20 ; C12N 1/00 ;
Abstract
The invention relates to the use of the regulatory subunit (R) of the cAMP dependent protein kinase (PKA) from for cAMP detection. It includes constructs for expression of the R-subunit in and fusion to green fluorescent proteins (GFP). Fluorescence energy transfer is used as a way to monitor cAMP binding, either by using fluorescently labelled cAMP or cGMP, or by using mutant GFPs with modified absorption and emission spectra. FRET changes upon cAMP binding will allow measurement of cAMP level either in vitro or within living cells.