The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 27, 2003
Filed:
May. 18, 1999
Growth state-specific immunofluorescent probes for determining physiological state and method of use
Applicant:
Inventor:
Paul Blum, Lincoln, NE (US);
Assignee:
Board of Regents of the University of Nebraska, Lincoln, NE (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 3/353 ; G01N 3/3554 ; G01N 3/3569 ; C12Q 1/10 ; C12N 1/509 ;
U.S. Cl.
CPC ...
G01N 3/353 ; G01N 3/3554 ; G01N 3/3569 ; C12Q 1/10 ; C12N 1/509 ;
Abstract
A novel method for examining bacterial growth state is carried out by measuring the levels of conserved cytosolic proteins specific for alternative growth states, using bacterial specific antibody fluorochrome-coupled probe. Utilizing the method of the invention, the cellular growth state of individual bacteria can be determined by measuring the abundance of growth state-specific protein homologs. For example, through use of the protein profiling method of the invention, bacterial VNC state can be distinguished by differentiating growing (exponential phase) from nongrowing or dormant (stationary phase) cells.