The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 13, 2003
Filed:
Oct. 27, 2000
Applicant:
Inventors:
Evelyn S. Sawyer, Arundel, ME (US);
Philip J. Sawyer, Arundel, ME (US);
Paul A. Janmey, Arundel, ME (US);
Assignee:
Sea Run Holdings, Inc., Freeport, ME (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 ;
U.S. Cl.
CPC ...
C12N 5/00 ;
Abstract
A method of using fish ovarian fluid for culture and preservation of mammalian cells includes obtaining ovarian fluid from a fish, and culturing mammalian cells in media including a commercially defined medium and a nutrient medium, wherein the nutrient medium includes the fish ovarian fluid. The cells may initially be cultured in a commercial medium and a conventional nutrient medium prior to culture in the fish ovarian fluid. Living cells may also be preserved by obtaining ovarian fluid from a fish, isolating the living cells, and adding the fish ovarian fluid to the living cells.