The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 13, 2003

Filed:

Nov. 12, 1996
Applicant:
Inventors:

Eric J. Beckman, Edgewood, PA (US);

Eliador J. Ghenciu, Pittsburgh, PA (US);

Nathaniel T. Becker, Burlingame, CA (US);

Landon M. Steele, Brisbane, CA (US);

Assignee:

Other;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 1/00 ; C12N 9/00 ; C12P 1/316 ;
U.S. Cl.
CPC ...
C07K 1/00 ; C12N 9/00 ; C12P 1/316 ;
Abstract

A method is provided for the extraction of water soluble biomaterials such as enzymes or proteins into carbon dioxide utilizing certain carbon dioxide soluble surfactants. The extraction can be performed on an aqueous solution, a fermentation broth or a fluid. The method includes the process steps of forming a carbon dioxide/surfactant mixture which involves dissolving carbon dioxide soluble surfactant(s) in carbon dioxide. The carbon dioxide can be in a liquid or supercritical form and the surfactant includes tail and head groups that interact with the biomaterials. Further, the mixture is added to the aqueous solution, fermentation broth or liquid under conditions to allow for extraction of the biomaterials. The method further includes depressurizing and/or temperature adjusting to remove the water soluble biomaterials. The surfactants include fluroethers, oligomers of propylene-oxide, siloxanes, etc. The biomaterials include proteins or enzymes. The carbon dioxide is suberitical or supercritical.


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