The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 15, 2003

Filed:

May. 21, 2002
Applicant:
Inventors:

Zaihui Zhang, Richmond, CA;

Jasbinder Sanghera, Vancouver, CA;

Shisen Wang, Coquitlam, CA;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/48 ; C12Q 1/42 ; C12Q 1/37 ; C12Q 1/00 ;
U.S. Cl.
CPC ...
C12Q 1/48 ; C12Q 1/42 ; C12Q 1/37 ; C12Q 1/00 ;
Abstract

An assay system is provided for detecting the enzymatic activity of a phosphorylation enzyme. The enzyme may be a phosphatase or protein kinase. The substrate for the enzyme is immobilized on a solid support via covalent or non-covalent binding through a signal enhancing polymer. The immobilized substrate provides an enhanced signal to background ratio when compared to a substrate in solution. The methods are easily adapted to high throughput screening systems.


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