The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 08, 2003
Filed:
Jun. 16, 2000
Christophe Beraud, San Francisco, CA (US);
Cara Ohashi, San Francisco, CA (US);
Roman Sakowicz, Foster City, CA (US);
Eugeni Vaisberg, Foster City, CA (US);
Ken Wood, Foster City, CA (US);
Ming Yu, Foster City, CA (US);
Cytokinetics, Inc., South San Francisco, CA (US);
Abstract
Described herein are methods of producing kinesins. In a preferred embodiment, the kinesins are produced from a prokaryote, most preferably, a bacterial cell. Bacterial expression offers several advantages over systems previously utilized, such as, for example, Bacculovirus. The yield of protein is higher, the cost of the expression setup is lower, and creation of alternative expression vectors is easier. The concern of copurifying a contaminating activity from the expression host is also eliminated since bacteria, in contrast to the bacculovirus expression system, do not have kinesin like proteins. Also described herein are purified kinesins, preferably unglycosylated and methods of use.