The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 6541256 B1

PDF
Full Text
Expired
Date of Patent:
Apr. 01, 2003

Filed:

Sep. 14, 2001
Applicant:
Inventors:

Ko-Hua Chen, Taipei, TW;

Nancy C. Joyce, Marlborough, MA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/00 ; C12N 5/02 ;
U.S. Cl.
CPC ...
C12N 5/00 ; C12N 5/02 ;
Abstract

A human cell growth medium formulation for culturing human cells of neural crest origin, most preferably corneal endothelial cells, or for accelerating the growth and proliferation of human cells of neural crest origin is disclosed. The formulation for the nutrient medium of the invention includes nerve growth factor, preferably at a concentration of 1-100 ng/ml and most preferably 20 ng/ml. The growth medium formulation preferably also includes epidermal growth factor (preferably at a concentration of 1-200 ng/ml and most preferably 5 ng/ml). Most preferably, the formulation of the invention also includes fibroblast growth factor (pituitary), at a concentration of 5-400 ng/ml or, most preferably, 40 ng/ml,


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