The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 25, 2003
Filed:
Jul. 30, 2001
David Nicholson Bell, Brampton, CA;
Danna Lynn Skea, Mississauga, CA;
Phyllis Robin Hedge, Cambridge, CA;
Hemosol Inc., Mississauga, CA;
Abstract
The method for obtaining and expanding TcR&ggr;&dgr; T cells in culture is described. The method involves: 1) culturing cells from a sample containing TcR&ggr;&dgr; T cells or precursors thereof in a first culture medium comprising a T cell mitogen and at least two cytokines and 2) culturing the cells obtained in step 1) in a second culture medium comprising at least two cytolines. Preferably, the method comprises 1) culturing the cells in a first culture medium comprising (a) a T cell mitogen, (b) interleukin-2 and (c) interleukin-4, and 2) culturing the cells obtained in step 1) in a second culture medium comprising (i) interleukin-2 and (ii) interleukin-4 to obtain TcR&ggr;&dgr; T cells. The TcR&ggr;&dgr; T cells obtained by the method can be used in a variety of experimental, therapeutic and commercial applications.