The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 25, 2003
Filed:
Jan. 11, 1999
Françoise Guillou-Bonnici, Villeurbanne, FR;
Eric Defranc, Goncelin, FR;
Antoine Hoang, Venissieux, FR;
Ali Laayoun, Lyons, FR;
Jean Lhomme, Meylan, FR;
Emmanuelle Trevisiol, Grenoble, FR;
Bio Merieux, Marcy l'Etoile, FR;
Abstract
A target nucleic acid sequence amplification method wherein at least the target nucleic acid sequence, at least one oligonucleotide primer specific for the target sequence, one or more enzymatic activities and nucleotides are provided, and the target sequence is amplified under conditions suitable in particular for said enzymatic or activity or activities, is disclosed. According to the method, at least one of the nucleotides is a prefunctionalized nucleotide that differs from the other nucleotides at least in that it includes at least one unprotected reactive covalence function in at least one predetermined site of the base of said nucleotide, in order to give a prefunctionalized amplificati product including at least one such prefunctionalized nucleotide. Nucleotides for carrying out the method are also disclosed.