The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 11, 2003

Filed:

Dec. 26, 2000
Applicant:
Inventors:

Santu Bandyopadhyay, Calcutta, IN;

Bikash Pal, Calcutta, IN;

Samir Bhattacharya, Calcutta, IN;

Mitali Ray, Calcutta, IN;

Keshab Chandra Roy, Calcutta, IN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 3/578 ;
U.S. Cl.
CPC ...
A61K 3/578 ;
Abstract

This invention relates to use of betel leaf extract to induce IFN&ggr; from human peripheral blood mononuclear cells and as a Th type immuno modulator, wherein the said method comprises the steps of, preparing water extract of betel leaf, preparation of human peripheral blood mononuclear cells, incubation of hPBMC with betel leaf extract for a period of 18-48 hours, extraction of RNA for cytokine specific RT-PCR or for flow cytometry for the detection of intracellular cytokine protein, subjecting RNA for RT-PCR to obtain PCR products using IFN&ggr; specific known primers, and enhancement of IFN&ggr; as reflected by IFN&ggr; specific band.


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