The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 04, 2003

Filed:

Apr. 07, 2000
Applicant:
Inventors:

Allan Svendsen, Birkerod, DK;

Torben Vedel Borchert, Copenhagen, DK;

Henrik Bisgard-Frantzen, Bagsvaerd, DK;

Helle Outtrup, Ballerup, DK;

Bjarne Ronfeldt Nielsen, Virum, DK;

Vibeke Skovgaard Nielsen, Bagsvœrd, DK;

Lisbeth Hedegaard, Skodsborg, DK;

Assignee:

Novozymes, A/S, Bagsvaerd, DK;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 2/106 ; C12N 9/00 ; C12N 9/24 ; C12N 9/28 ; C12N 1/20 ; C12N 1/500 ; C02H 2/104 ;
U.S. Cl.
CPC ...
C12P 2/106 ; C12N 9/00 ; C12N 9/24 ; C12N 9/28 ; C12N 1/20 ; C12N 1/500 ; C02H 2/104 ;
Abstract

The invention relates to a novel Termamyl-like &agr;-amylase, and Termamyl-like &agr;-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an &agr;-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an &agr;-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an &agr;-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an &agr;-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like &agr;-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca concentrations (relative to the parent).


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