The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 18, 2003
Filed:
Mar. 09, 2000
Applicant:
Inventors:
Ronald Laskey, Cambridge, GB;
Gareth Haydn Williams, Cambridge, GB;
Torsten Krude, Cambridge, GB;
Kal Stoeber, Cambridge, GB;
Anthony David Mills, Cambridge, GB;
Assignee:
Cancer Research Campaign Technology Limited, London, GB;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12P 1/934 ; C12N 1/38 ; C12N 5/00 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12P 1/934 ; C12N 1/38 ; C12N 5/00 ;
Abstract
The invention relates to improvements in cell-free systems for initiating DNA replication under cell cycle control. The system comprises a synchronous population of G1 nuclei which have been released from G0; and S phase cytosol. In a preferred aspect, a polypeptide is supplied to the system, wherein the polypeptide is Cdc6 and/or at least one MCM protein. The system is suitable for DNA replication assays, for example to test substances which modulate DNA replication.