The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 07, 2003

Filed:

Mar. 21, 2001
Applicant:
Inventors:

Guohan Yang, Mansfield, MA (US);

Donna M. Ford, Plainville, MA (US);

Say-Jong Law, Westwood, MA (US);

John E. Monahan, Walpole, MA (US);

Todd B. Sells, Bellingham, MA (US);

Assignee:

Bayer Corporation, Medfield, MA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07H 2/104 ; C07H 2/102 ; C12A 1/68 ; C07D / ;
U.S. Cl.
CPC ...
C07H 2/104 ; C07H 2/102 ; C12A 1/68 ; C07D / ;
Abstract

Highly hydrophilic non-nucleosidic tags with multiple labels are provided for use in nucleic acid probes. The tags are branched structures having a phosphodiester backbone, which have the advantages of a small dimensional size and high hydrophilicity. After the tag is labeled, its high negative charge and minimal size help to keep the carriers away from DNA or RNA molecules, due to repulsion between negative charges. Non-specific intercalation and steric hindrance are therefore minimized, and the hydrophobicity, if any of reporter molecules is reduced. The probes are used in place of conventionally labeled oligonucleotides for a variety of hybridization reactions.


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