The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 31, 2002
Filed:
Dec. 03, 1998
Joseph D. Andrade, Salt Lake City, UT (US);
Chung-Yih Wang, Midvale, UT (US);
Vladimir Hlady, Salt Lake City, UT (US);
Philip M. Triolo, Salt Lake City, UT (US);
Robert J. Scheer, Salt Lake City, UT (US);
University of Utah Research Foundation, Salt Lake City, UT (US);
Abstract
A method and associated apparatus for measuring chemical concentration in a liquid sample based on spatial separation and resolution of light is disclosed. The method is preferably applied to sensitive, quantitative, luminescence-based biosensors which reads the analyte concentration via spatial distribution of the emitted light. The detection of light is used to assess the spatial position, rather than the intensity or wavelength, of emitted light. A bioluminescent or chemiluminescent reaction requiring, for example, ATP, NADPH or NADH as a specific, and sensitive co-factor is used. ATP or NADH concentration is modulated, “tuned,” and/or regulated via, for example, an enzyme which consumes (“consumase”) ATP, NADPH, or NADH, thereby producing a spatial distribution of ATP or NADH and a spatial distribution in the emitted light. By appropriate control of the consumase or “synthase” activity and kinetics, a sensitive, specific, and easily readable luminescent pattern is produced, permitting detection.