The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 31, 2002

Filed:

May. 05, 2000
Applicant:
Inventors:

Gopalan Selvaraj, Saskatoon, CA;

Ramesh B. Nair, Saskatoon, CA;

Richard W. Joy, IV, Alix, CA;

Wilfred A. Keller, Saskatoon, CA;

Raju S. Datla, Saskatoon, CA;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A01H 5/00 ; C12N 1/300 ; C12N 1/500 ; C12P 2/106 ; C07H 2/104 ;
U.S. Cl.
CPC ...
A01H 5/00 ; C12N 1/300 ; C12N 1/500 ; C12P 2/106 ; C07H 2/104 ;
Abstract

The sinapine content of seeds of , and other crucifera plants, and the resulting seed meal made therefrom, is reduced by transforming cells of the plants to incorporate an expressible exogenous CYP84 monooxygenase enzyme, particularly ferulate 5-hyroxylase (F5H:) enzyme, or an antisense equivalent thereof. This allows for the production of a seed meal that is commercially more valuable. Three specific nucleic acid sequences encoding the F5H polypeptide are disclosed, designated BNF5H1, BNF5H2 and BNF5H3, and genetic constructs produced. The antisense suppression of sinapine is preferred, which can reduce the sinapine content of seed meal by up to 40% compared to wild type or vector-only transformed plants.


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