The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 10, 2002

Filed:

Dec. 09, 1999
Applicant:
Inventors:

Toshinari Sakurai, Hitachinaka, JP;

Shinichi Fukuzono, Hitachinaka, JP;

Kenji Yasuda, Tokyo, JP;

Assignee:

Hitachi, Ltd., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/00 ; C12M 3/00 ; C12N 1/102 ; C07H 2/100 ;
U.S. Cl.
CPC ...
C12M 1/00 ; C12M 3/00 ; C12N 1/102 ; C07H 2/100 ;
Abstract

Methods and apparatus are provided for the recovery of nucleic acids from a nucleic acid-containing material by which nucleic acids can be rapidly and easily recovered at a high purity without deteriorating the yield. The methods are composed of step 1 for promoting the release of nucleic acids from a nucleic acid-containing material, step 2 for mixing the released nucleic acids with an accelerator substance for the binding of nucleic acids to a solid phase, step 3 for bringing the mixture in contact with a solid phase bondable to nucleic acids, step 4 for isolating the solid phase from a liquid, step 5 for washing the solid phase with a solution containing a salt, and a step 6 for eluting the nucleic acids from the solid phase. Accordingly, nucleic acids at a suitable purity for genetic tests or gene analyses can be rapidly and easily recovered without the use of hazardous substances. The apparatus may contain a reactor, at least one pipette, a purified product container, transport mechanisms for the reactor and purified product container, motors to control the pipette and transport mechanisms, a mechanical control unit to control the motors, and a computer that controls the mechanical control unit.


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