The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 03, 2002
Filed:
Apr. 18, 2001
Applicant:
Inventors:
Kazuhiko Shimada, Tokuyama, JP;
Yuji Setoguchi, Shinnanyo, JP;
Kazuyuki Oishi, Tokuyama, JP;
Toshiki Kawabe, Shiga, JP;
Assignee:
Sekisui Chemical Co., Ltd., Osaka, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 1/508 ;
U.S. Cl.
CPC ...
B01D 1/508 ;
Abstract
A method for separating hemoglobin A from a hemoglobin mixture according to a cation exchange liquid chromatography characterized that at least two types of eluents are used including an eluent (hereinafter referred to as “eluent A”) for elution of faster hemoglobins than hemoglobin A and an eluent (hereinafter referred to as “eluent B”) for elution of hemoglobin A and slower hemoglobins than hemoglobin A , and that the eluent A has a pH of 4.0-6.0 and the eluent B is maintained at a pH of at least 0.5 higher than that of the eluent A.