The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 12, 2002
Filed:
May. 16, 2000
Applicant:
Inventor:
Simon Delagrave, Avondale, PA (US);
Assignee:
Hercules, Incorporated, Wilmington, DE (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 1/934 ; C12Q 1/68 ; C07H 2/100 ; C07H 2/102 ; C07H 2/104 ; C07H 1/904 ; G01N 3/3566 ;
U.S. Cl.
CPC ...
C12P 1/934 ; C12Q 1/68 ; C07H 2/100 ; C07H 2/102 ; C07H 2/104 ; C07H 1/904 ; G01N 3/3566 ;
Abstract
The present invention provides methods of preparing large polynucleotides of arbitrary sequence and in a manner that will readily lend itself to automation. The present invention provides methods of preparing a polynucleotide having at least 200 nucleotides in either a 5′ to 3′ or 3′ to 5′ direction by ligating a plurality of oligonucleotides, the assembly of which, represents the nucleotide sequence of the desired polynucleotide.