The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 05, 2002
Filed:
Jun. 07, 1995
Howard Benjamin, Lexington, MA (US);
Ethan Signer, Cambridge, MA (US);
Malcolm Gefter, Lincoln, MA (US);
Praecis Pharmaceuticals, Inc., Waltham, MA (US);
Abstract
An anchor library is described. A collection of recombinant vectors having a nucleic acid encoding a displayed peptide sequence is provided. The displayed peptide sequence of each of the vectors comprises X (Y ) X (Y ) X (Y ) X , wherein each X , X , X and X is an amino acid residue and any of X , X , X and X can be the same or different from any one other, wherein each Y , Y and Y is alanine or glycine or a combination of alanine and glycine that is respectively c , c and c amino acid residues long and any of Y , Y and Y if present can be the same or different from any one other, wherein each of c , c and c is 0 to about 20, wherein X and X are each attached to an amino acid residue that flanks the displayed peptide sequence. Preferably, at least about 10 to about 10 permutations of all possible permutations of the displayed peptide sequence are present in the anchor library. Preferably, the library does not contain more than about 10% of displayed peptide sequences different from the first mentioned displayed peptide sequences. Also described are methods of making anchor libraries and methods of using anchor libraries to identify a peptide sequence that binds to a target. Recombinant vectors, filamentous phage, nucleic acid molecules and proteins are also provided.