The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 05, 2002
Filed:
Jun. 11, 2001
Applicant:
Inventors:
Claudio Bordignon, Milan, IT;
Bernhard Goller, Penzberg, DE;
Ruediger Rueger, Huglfing, DE;
Georg Tiefenthaler, Sindelsdorf, DE;
Assignee:
Roche Diagnostics GmbH, Mannheim, DE;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/00 ;
U.S. Cl.
CPC ...
C12N 5/00 ;
Abstract
A titer of retroviral vectors of at least about 10 colony-forming units/ml cell culture supernatants is obtained using an amphotropic retroviral packaging cell line which contains functional gag, pol and env genes integrated into the genome in which the expression of the genes gag and pol is regulated independently of the expression of the env genes, wherein the genome of the said cell line contains one or two functionally active env genes.