The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 05, 2002
Filed:
May. 27, 1999
Werner Baumann, Freiburg, DE;
Ralf Ehret, Merdingen, DE;
Mirko Lehmann, Freiburg, DE;
Günter Igel, Teningen, DE;
Hans-Jürgen Gahle, Emmendingen, DE;
Bernhard Wolf, Stegen, DE;
Ulrich Sieben, Reute, DE;
Ingo Freund, Vogtsburg-Oberrotweil, DE;
Martin Brischwein, Freiburg, DE;
Micronas GmbH, Freiburg, DE;
Abstract
A process is provided for the intracellular manipulation of a biological cell ( ) which is positioned adhering to a support area ( ) in a culture medium ( ). Inside the support area ( ) for the cell ( ) an opening into the membrane of the cell ( ) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area ( ), thus seals off the cell fluid situated in the interior of the cell ( ) from the culture medium ( ) and insulates the cell fluid against the culture medium ( ). The interior of the cell ( ) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier ( ) with a support area ( ) for adhering the cell and a poration tool ( ) for creating the opening in the cell membrane. The poration tool ( ) may be any of various chemical, mechanical and/or electrical devices.