The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 6475389 B1

PDF
Full Text
Expired
Date of Patent:
Nov. 05, 2002

Filed:

May. 08, 2001
Applicant:
Inventors:

Elzbieta Kawinski, Orchard Park, NY (US);

Kailash C. Chadha, Williamsville, NY (US);

Assignee:

Health Research, Inc., Buffalo, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 3/3574 ;
U.S. Cl.
CPC ...
G01N 3/3574 ;
Abstract

A method for detecting prostate specific membrane antigen (PSMA) in serum by immunoassay including the steps of capturing PSMA from serum using a PSMA antibody and detecting the captured PSMA with an anti-&agr; -antichymotrypsin antibody. In a preferred embodiment, PSMA is captured using a PSMA antibody by diluting the PSMA antibody, coating a surface such as the surface of wells in a microtiter plate, with the resulting solution, blocking non-specific sites on the surface with albumin, diluting the serum with a blocking buffer and applying a diluted sample to the surface to capture PSMA. A preferred embodiment for detecting captured PSMA, includes the steps of removing non-captured material by washing the surface, applying anti-&agr; antichymotrypsin antibody diluted in a blocking solution such as albumin solution and detecting bound PSMA using peroxidase labeled anti-rabbit IgG and OPD substrate.


Find Patent Forward Citations

Kawinski, Elzbieta. (2002). Method for detection of prostate specific membrane antigen in serum (U.S. Patent No. 6475389). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/6475389

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