The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 15, 2002

Filed:

Jun. 30, 2000
Applicant:
Inventors:

Dennis Murphy, Paoli, PA (US);

John Reid, Bryn Mawr, PA (US);

Dan Robertson, Haddonfield, NJ (US);

Assignee:

Diversa Corporation, San Diego, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/34 ; C12N 9/78 ; C12N 9/80 ; C12N 1/20 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12Q 1/34 ; C12N 9/78 ; C12N 9/80 ; C12N 1/20 ; C07H 2/104 ;
Abstract

A purified thermostable enzyme is derived from the archael bacterium Thermococcus GU5L5. The enzyme has a molecular weight of about 68.5 kilodaltons and has cellulase activity. The enzyme can be produced from native or recombinant host cells and can be used for the removal of arginine, phenylalanine, or methionine amino acids from the N-terminal end of peptides in peptide or peptidomimetic synthesis. The enzyme is selective for the L, or 'natural' enantiomer of the amino acid derivatives and is therefore useful for the production of optically active compounds. These reactions can be performed in the presence of the chemically more reactive ester functionally, a step which is very difficult to achieve with nonenzymatic methods.


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