The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 01, 2002
Filed:
Dec. 01, 2000
Andrew P. Miller, San Diego, CA (US);
DNA Sciences, Inc., Fremont, CA (US);
Abstract
The present invention provides methods and kits for determining the identity of a nucleotide at a variant site on a target nucleic acid. The methods begin with the template-dependent amplification of a target sequence under defined conditions to achieve selective incorporation of a nucleotide analog at the variant site. Amplification product is then subjected to limited degradation to create products having allele-specific sizes, which are subsequently separated on the basis of size. Finally, the number of products and their sizes is to assessed to determine the identity of the nucleotide(s) at the variant site and the genotype of the organism from which the target was obtained.