The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 20, 2002
Filed:
Jul. 26, 2000
Hauke Lilie, Halle, DE;
Susanne Richter, Halle, DE;
Rainer Rudolph, Halle, DE;
Kay-Gunnar Stubenrauch, Munich, DE;
Hoffmann-La Roche Inc., Nutley, NJ (US);
Abstract
A chimeric polypeptide has a first and a second polypeptide chain chemically linked via 1 to 3 cysteine-based disulfide bridges. The first polypeptide chain consists of 1 to 3 cysteines and 4 to 12 basic amino acids preferably selected from the group consisting of arginine, lysine and ornithine. The second polypeptide chain consists of 1 to 3 cysteines and 4 to 12 acidic amino acids selected from the group consisting of glutamate and aspartate. Each polypeptide chain is linked at its C- or N-terminus to a biologically active compound, and is useful as a multimeric pharmaceutical agent. A method for producing such chimeric polypeptide involves contacting the first polypeptide chain with the second polypeptide chain under conditions such that the basic amino acids on the first polypeptide chain ionically interact with the acidic amino acids on the second polypeptide chain and then treating the first polypeptide chain and the second polypeptide chain with an oxidizing agent under conditions such that the 1 to 3 cysteines of the first polypeptide chain form disulfide bridges with the 1 to 3 cysteines of the second polypeptide chain thereby forming the chimeric polypeptide.