The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 06, 2002

Filed:

Jan. 26, 2001
Applicant:
Inventor:

Stephen F. Hardy, San Francisco, CA (US);

Assignee:

Chiron Corporation, Emeryville, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 7/00 ; C12Q 1/68 ; C12P 2/106 ; C07H 2/104 ; A61K 3/912 ;
U.S. Cl.
CPC ...
C12N 7/00 ; C12Q 1/68 ; C12P 2/106 ; C07H 2/104 ; A61K 3/912 ;
Abstract

Methods and compositions are provided for producing recombinant AAV vector particles; comprising the general steps of (a) introducing into a host cell (i) pfloxAAV, (ii) a recombinant viral vector encoding plasmid, and (iii) a plasmid encoding herpesvirus, cytomegalovirus, or adenoviral functions, or a herpesvirus, cytomegalovirus, or, adenovirus itself, in order to produce flox AAV particles and recombinant AAV particles; and (b) introducing into a second host cell (i) the recombinant AAV particles and flox AAV particles of (a), (ii) a vector which directs the expression of Cre, and (iii) a vector which directs the expression of herpesvirus, CMV, or adenovirus helper functions, such that said recombinant AAV vector particles are produced.


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