The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 23, 2002
Filed:
Oct. 14, 1999
Applicant:
Inventor:
Stuart Harbron, Berkhamsted, GB;
Assignee:
Zetatronics Limited, London, GB;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12N 9/22 ; C12N 1/20 ; C12P 1/934 ; C07A 2/102 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12N 9/22 ; C12N 1/20 ; C12P 1/934 ; C07A 2/102 ; C07H 2/104 ;
Abstract
A method is disclosed for detecting single-stranded target nucleic acid ( ) which comprises the steps of forming a hybrid between said target nucleic acid and a nucleic acid probe ( ), said nucleic acid probe labelled with an enzyme reagent ( ) which hydrolyses single-stranded nucleic acid but is substantially without effect on double-stranded nucleic acid, said hybrid formed under conditions of pH which are outside the activity range of said enzyme reagent, adjusting said pH to a value within the activity range of said enzyme reagent allowing said enzyme reagent substantially to hydrolyse any single-stranded nucleic acid present, and detecting said hybrid.