Optical molecular sensors for cytochrome p450 activity

Abstract

The invention provides a compound, useful as an optical probe or sensor of the activity of at least one cytochrome P450 enzyme, and methods of using the compound to screen candidate drugs, and candidate drugs identified by these methods. The optical probe of the invention is a compound having the generic structure Y—L—Q, wherein Y is selected from the group consisting of Q as herein defined, saturated C -C alkyl, unsaturated C -C alkenyl, unsaturated C -C alkynyl, substituted saturated C -C alkyl, substituted unsaturated C -C alkenyl, substituted unsaturated C -C alkyl, C -C cycloalkyl, C -C cycloalkenyl, substituted saturated C -C cycloalkyl, substituted unsaturated C -C cycloalkenyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl; L is selected from the group of (—OCR H) —, wherein for each p, all R are separately selected from the group consisting of a hydrogen atom, saturated C -C alkyl, unsaturated C -C alkenyl, unsaturated C -C alkynyl, substituted saturated C -C alkyl, substituted unsaturated C -C alkenyl, substituted unsaturated C -C alkyl, C -C cycloalkyl, C -C cycloalkenyl, substituted saturated C -C cycloalkyl, substituted unsaturated C -C cycloalkenyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, and p is a positive integer no greater than twelve; and Q is a chemical moiety that gives rise to optical properties in its hydroxy or hyrdoxylate, phenol or phenoxide form that are different from the optical properties that arise from its ether form. Most preferably, p is one, R is hydrogen, and Q is the ether form of a phenoxide fluorophore.