The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 18, 2002
Filed:
Jun. 22, 2000
Applicant:
Inventors:
Rakesh Tuli, Lucknow, IN;
Samir V. Sawant, Lucknow, IN;
Assignee:
Council of Scientific and Industrial Research, New Delhi, IN;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12N 1/564 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12N 1/564 ;
Abstract
The present invention relates to a process for uniform and efficient delivery of metal projectiles into living cells and tissue of plants and animals, by bombarding the cells with biological coated on metal bead particles. The metal bead particles are heated in dry oven to high temperature, prior to coating with DNA and by substituting ethanol with isopropanol while coating the particles with DNA, to give several fold higher expression of the nucleic acid delivered into cells.