The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 11, 2002

Filed:

Dec. 22, 1999
Applicant:
Inventors:

Jing Cheng, Bejing, CN;

Lei Wu, San Diego, CA (US);

Michael J. Heller, Encinitas, CA (US);

Ed Sheldon, Arcadia, CA (US);

Jonathan Diver, San Diego, CA (US);

James P. O'Connell, Solana Beach, CA (US);

Dan Smolko, Jamul, CA (US);

Shila Jalali, San Diego, CA (US);

David Willoughby, San Diego, CA (US);

Assignee:

Nanogen, Inc., San Diego, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/34 ;
U.S. Cl.
CPC ...
C12M 1/34 ;
Abstract

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.


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