The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 04, 2002
Filed:
Jun. 23, 2000
Yoshimasa Saito, Kawanishi, JP;
Yuji Noguchi, Aichi, JP;
Koji Yoshikawa, Ushiku, JP;
Shinsuke Soeda, Nagoya, JP;
Fujisawa Pharmaceutical Co., Ltd., Osaka, JP;
Abstract
A DNA derived from plasmid pF4, which contains a region involved in the control of autonomous replication in a bacterial cell belonging to the genus Gluconobactor, with or without a part of the polynucleotide region not essential for the autonomous replication therein, particularly, the same DNA comprising, as the region involved in the control of autonomous replication in the bacterial cell belonging to the genus Gluconobactor, a part or the entirety of the polynucleotide of nucleotide No. 2897-3969 region of Sequence Listing SEQ:ID No. 1. A plasmid vector containing this DNA, transformant transformed with this plasmid vector, and a method for producing a physiologically active substance comprising culturing this transformant. The full length nucleotide sequence of plasmid pF4 and the region containing DNA involved in the control of automonous replication in the bacterial cell belonging to the genus Gluconobactor were specified, whereby the region not essential for the automonous replication could be removed to provide a shortened pF4. The shortening of the region in the vector for autonomous replication increases the remaining region of the vector and allows incorporation of many structural genes into the vector. Consequently, the vector can have many functions.