The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 21, 2002
Filed:
Apr. 14, 1998
Michael A. Resnick, Chapel Hill, NC (US);
Vladimir L. Larionov, Chapel Hill, NC (US);
Natalay Y. Kouprina, Chapel Hill, NC (US);
Edward L. Perkins, Sunnyvale, CA (US);
Abstract
The invention is directed to a method of making a yeast artificial chromosome (YAC) comprising introducing into yeast cells a population of nucleic acids and a vector, wherein the vector comprises a yeast centromere, a selectable marker, a yeast telomere, and a sequence which can recombine with a region of a nucleic acid within the population of nucleic acids, whereby in vivo recombination makes the YAC. The invention is also directed to a method of making a YAC using two vectors and a method of making a circular YAC. The invention is also directed toward methods of making YACs with a selected nucleic acid insert from a mixed population of nucleic acids using transformation-associated recombination. The invention is further directed toward a method of cloning a selected nucleic acid from a population of nucleic acids into a vector comprising introducing into yeast cells a population of nucleic acids and the vector, wherein the vector comprises a specific sequence which can recombine with a region of the selected nucleic acid within the population of nucleic acids and a non-specific sequence which can recombine with the selected nucleic acid within the population of nucleic acids; whereby in vivo recombination makes a clone of the vector selected nucleic within the vector. The invention is also directed toward the products made by, and the vectors and reagents used in these methods. The invention is also directed toward a method of TAR cloning in
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